iGEM国际基因工程机器大赛辅导-翰林国际教育

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翰林国际学术活动流程

2022iGEM国际基因工程机器大赛辅导

一个关于合成生物学的世界顶级赛事

为什么参加IGEM?

全球最高水平的全成生物学学术学术活动
最受美国名校青睐的学术学术活动之一
学术活动机制涵盖生物、数学、计算机、机械电子工程设计等多学科
与世界各地名校队伍同台竞技，全面提高综合素质，开拓国际视野
浙大、武大等顶尖双一流高校教授全程指导
专职名校博士团队倾力带队指导

IGEM学术活动辅导

1.基础教学

（1）科学思维与学术研究导论
（2）合成生物学及基理论基础课程
（3）IGEM学术活动规则指导
（4）IGEM学术活动团队领导力课程（中学基因工程学术社团指导）
（5）基因工程基础实验
（6）生物信息学习与数学建模

2.实验指导

（1）命题讨论与聚焦
（2）命题设计与实验设计指导
（3）实验操作指导
（4）数据处理与建模指导
（5）论文写作指导
（6）学术答辩指导

3.社会活动
（1）课题的公众宣传
（2）社会调研（企业、社区）
（3）学者调研（教授、院士、医生）
（4）与其他参赛队的互助实验
（5）赞助方案与众筹指导

4.项目展示
（1）海报、PPT设计
（2）项目展示页面（WIKI）设计
（3）演讲与答辩技巧指导

参赛指导
（1）赴美参赛的后勤保障
（2）比赛临场指导

iGEM参赛时间安排表

时间	内容
1-2月	赛前冬令营集训
3月	每周末线上培训，讲解近期论文和往届优秀项目。组织1次线下周末活动，探讨项目，撰写可行性方案。设计第一批生物砖。
4月	周末线上培训。文献阅读和讨论。组织1次周末实验，确定项目，确定DNA parts，订购引物。进行预实验构建第一批生物砖。
5月	每周末线上交流，文献阅读和讨论。查阅资料，完善项目背景。开展有关项目背景的human practice 组织1次周末实验，测试实验条件。
6月	每周末线上交流，文献阅读和讨论。暑假前，组织学生1-2次周末实验，获取数据。
7月	进实验室做实验，获取实验数据。进行数学建模训练。
8月	进实验室做实验，获取实验数据。组织学生参与队伍交流。
9月	协助学生整理实验数据，完成wiki框架和背景内容。完成ppt草稿进行与项目展示相关的human practice
10月	每周线上交流。整理wiki，poster，训练ppt展示。比赛前邀请优秀的科学家评委，安排1-2次ppt答辩
11月	赛前准备，赴美比赛

申请条件

（1）高一、高二在校学生（每队8-15名）
（2）具备良好的英文听说读写能力
（3）具有团队沟通、协作与表达能力
（4）对研究性学习有着浓厚的兴趣、希望培养自已独立思考、分析与解决问题的能力
（5）有一定的实验、美术设计、编程、建模、摄影、英文等基础将成为加分项

报名及面试流程

1.学生填写报名表。
2.导师对申请者进行审核。通过审核后，学生需参加面试。
3.学生通过面试后签订科研指导协议，并支付参赛辅导费、报名费、实验室使用费、海外参赛费。
4.付费后，学员将收到录取通知、实验室报到通知，课程计划以及预习资料包。

2018-2019IGEM获奖作品部分展示（共9队）

1. 队名：Great Bay China

项目名称：microbial Compartmentalization AssisTed Nepetalactol Ingredient Production

奖项：

（1）Grand Prize Winner
（2）Best Wiki
（3）Best Poster
（4）Best Model
（5）Best New Composite Part
（6）Best Part Collection
（7）Best Product Design
（8）Best New Basic Part

摘要/项目介绍：

Nepetalactone is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between E. coli and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE)stabilized promoters to regulate the heterologous gene expression in E. coli. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.

2. 队名：GreatBay_SZ

项目名称：SPIDERMAN_SPIDroin EngineeRing with chroMoprotein And Natural dye

奖项：

（1）Grand Prize Winner;
（2）Best Education and Public Engagement;
（3）Best Hardware;
（4）Best Integrated Human Practices;
（5）Best Measuremen;
（6）Best Model;
（7）Best New Basic Part;
（8）Best New Composite Part;
（9）Best Part Collection;
（10）Best Poster;
（11）Best Presentation;
（12）Best Wiki;
（13）iGEMers' Prize

摘要/项目介绍：

Spider silk serves as a new material with superior properties that can be applied in medication, cloth, and aerospace fields. However, spider breeding is not applicable due to spider's fierce behavior. The current approach is to produce recombinant spidroins (silk proteins) from other chassis and spin them into silk. This year , we aim to manufacture recombinant spider silk with E.coli and color the silk for application in cloth industry. We modularized three significant domains of spidroin - the N-terminal, the repetitive region, and the C-terminal - and integrated them into various spidroin to form silk. We then dyed the silk with microbial natural pigments deoxyviolacein and indigo. To obtain better color and a more convenient dying process, we fused the repetitive region to chromoproteins and mixed them with spidroin during spinning. Our team hopes to provide a novel approach for cloth production and explore new possibilities for spider silk applications.

3. 队名：Mingdao

项目名称：Fresh Air, the clever heir

奖项：

（1）Best Education and Public Engagement;
（2）Best Integrated Human Practices;
（3）Best Model;
（4）Best New Basic Part;
（5）Best New Composite Part;
（6）Best Part Collection;
（7）Best Poster;
（8）Best Supporting Entrepreneurship;
（9）Best Wiki

摘要/项目介绍：

To increase our algae system's efficiency of reducing indoor pollutants such as CO2 and VOCs, we applied two enzymes, carbonic anhydrase(CA) and cytochrome P450 2E1(CYP2E1) to improve algal absorption of CO2 and VOCs, respectively. We produced enzymes by engineering Bacillus subtilis 168 with BioBricks we created and then finally put them into our system. We also did some experiments to analyze the activity of our enzymes and test whether our system is efficient enough to refresh indoor air.

4. 队名：GENAS_China

项目名称：Recombinase-Based Biological Relay devices (RBBR),

奖项：

（1）Best Measuremen;
（2）Best Model;
（3）Best New Basic Part;
（4）Best Part Collection;Best Wiki

摘要/奖项介绍：

Generally, the relay, the key component of electrical automatic control system, receives the output signal of a control module and thus shifts the ON/OFF state of a separated working module. Based on the recombinase-attB/attP system and unidirectional terminator, we constructed a set of orthogonal Recombinase-Based Biological Relay devices (RBBR), whose response intervals were characterized by accurate quantifying method so that they can be predictably adapted to different genetic circuits. We designed and constructed a resolution extensible analog-digital converter (ADC), which converts the consecutive analog quantities (the strength of an inducible promoter) into discrete digital signals (indicated by different chromoproteins), allowing the digitized processing and storage of signals. Beyond the common use of recombinase system as simple response to two input levels, our project achieves modifying and utilizing the response interval of this system. The application of relay in genetic circuit can contribute to the improvement of the modularity of artificial biological system.

5. 队名：US_AFRL_CarrollHS

项目名称：ENGINEERING A CELL-FREE HEXAVALENT CHROMIUM DETECTION SYSTEM

奖项：Best Education and Public Engagement

摘要/项目介绍：

Methylophaga flavin-containing monooxygenase (bFMO) is the gene responsible for the conversion of the Indole into Isatin, which is then catalyzed into indigoid compounds. These compounds display an indigo color which can be easily detected (KAIST). Our project will focus on using bFMO as indicator to determine when toxic hexavalent chromium is present. The bFMO will be cloned into the C4-HSL circuit created by our team last year, in order to show the indigo color can be produced and easily seen. This will be done by replacing GFPa1 (green fluorescent protein) with the bFMO gene. Next, bFMO will be cloned into a construct with a T7 promoter, which we believe will be the most efficient promoter for bFMO. This construct will then be tested to ensure that the promoter will produce bFMO. Once this is done, the chrB repressor will be introduced. In order to determine when hexavalent chromium is present, a repressor, chrB, will be cloned into a construct along with the promoter, chrP, associated with the repressor. This construct will then be cloned into a pet11a backbone, which already has both the T7 promoter and rbs needed. The plasmid should then produce chrB, which will disrupt the chrP causing bFMO to not be produced. A commercial cell-free extract kit will then be used on this construct, and the T7-chrB plasmid will be added in order to make ChrB. When chromium is detected the ChrB repressor will be ‘knocked off,’ allowing the promoter to function and bFMO to catalyze into indigoid compounds, emitting a detectable indigo color.

6. 队名：SHSBNU_China

项目名称：/

奖项：Best New Composite Part

摘要/项目介绍：

DNA is a biological macro-molecule which can carry huge amount of information accurately, and this feature can be used to achieve data recordings in vivo. Our project aims to build a biological recorder that can monitor extracellular information and record it on DNA. Recombinase or CRISPR base-editor is used to target specific DNA addresses and generate mutations in a reporter gene, so the recordings can be quantitatively measured to infer intensity and duration information about the chemicals of disease, such as inflammatory bowel disease. Additionally, we designed a hardware for in situ biomolecular detection to monitor gastrointestinal health. This platform could enable more precise detection and could help improve the management and diagnosis of gastrointestinal disease.

7. 队名：Greatbay_SCIE

项目名称：Stickit

奖项：Best Part Collection

摘要/项目介绍：

To create a well-functioning adhesive, cohesion--the ability to maintain the shape of the material--and adhesion--the ability to stick to underwater surfaces--should cooperate. Our toolbox consists of several groups of biological parts from marine creatures (mussel and barnacles) and prokaryotes (E.coli MG1655 and Marine Archaea). These proteins will provide cohesion, adhesion, and other special qualities. We will then combine these proteins using principles of synthetic biology to create protein adhesives with high-performance and biocompatibility. Their potentials are limitless and can be applied to many fields.

8. 队名：TAS_Taipei

项目名称：ADIEU, RESIDUES!

奖项：Best Part Collection

摘要/项目介绍：

We consume fruits and vegetables every day without knowing whether or not agricultural residues (i.e. pesticides & heavy metals) are present. In 2017, according to the UN, exposure to pesticides caused 200,000 to 300,000 deaths annually. Additionally, lead exposure alone is responsible for a death rate of 25.3 per 100,000 individuals in East Asia. Current methods of agricultural residue detection are not easily accessible to the public and are inconvenient for everyday use. Thus, our project aims to allow for convenient visualization of agricultural residues by designing colored proteins that can directly interact with these residues. We envision a system where our designed proteins can be applied directly on food items to detect the presence of residues. Our final product can be used by consumers, distributors and farmers alike.

9. 队名：TPHS_San_Diego

项目名称：Breakdown of Trimethylamine via Trimethylamine Dehydrogenase to Minimize Heart Disease Caused by Red Meat Consumption

奖项：Best Plant Synthetic Biology

摘要/项目介绍：

The consumption of red meat has been linked to atherosclerosis, a form of heart disease caused by the buildup of plaque in the arteries. Recently, it was discovered that the combination of choline and L-carnitine from red meat, are converted to trimethylamine (TMA) in the body. TMA is a precursor to trimethylamine N-oxide (TMAO), which is found to exacerbate cholesterol buildup, ultimately leading to atherosclerosis. In this study, we targeted this pathway by breaking down the precursor, TMA, before TMAO is formed. A system designed to concurrently degrade TMA and subdue its toxic by-product, formaldehyde, was implemented in E. coli for these purposes. This system is composed of Trimethylamine Dehydrogenase (TMADH) and Formaldehyde Dehydrogenase (FDH) which are being tested independently for their degradation properties. Our approach has proven that TMADH is effective in TMA degradation and FDH can be expressed in a bacterial vector to minimize the presence of formaldehyde.