The graph below shows the results of an enzyme rate reaction. Using this graph, calculate the initial rate of reaction.
Step 1: Estimate the extrapolated curve of the graph
Step 2: Find the tangent to the curve at 0 seconds (the start of the reaction)
The tangent drawn in the graph above shows that 72 cm3 of product was produced in the first 20 seconds.Step 3: Calculate the gradient of the tangent (this will give you the initial rate of reaction):
Gradient = change in y-axis ÷ change in x-axis
Initial rate of reaction = 72 cm3 ÷ 20 s
Initial rate of reaction = 3.6 cm3 s-1
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