2018英特尔ISEF学科最佳作品摘要：细胞与分子生物学 | Best of Category Abstract: CELLULAR AND MOLECULAR BIOLOGY

获奖作品基本信息

年份	2018
学科	细胞与分子生物学 CELLULAR AND MOLECULAR BIOLOGY
学校	Horace Mann School
国家/州	NY，United States of America

获奖作品名称

Exploring Posterior Growth in D. rerio Using a Live Cell Cycle Biosensor

获奖作品摘要

Though cell cycle regulation plays a key role in numerous developmental processes, including morphogenesis and differentiation, the regulatory systems that control the cell cycle remain poorly understood. Surprisingly, past studies have shown a correlation between invasive behavior and G1 cell cycle arrest. To explore this relationship, a cell cycle state biosensor has been adapted for use in zebrafish. The sensor provides a visual readout of cell cycle state based on the localization of DNA Helicase B (DHB), which is determined by cyclin-dependent kinase 2 (CDK2) activity. The sensor was validated by comparing it with Fluorescence Ubiquitin Cell Cycle Indicator (Fucci) and characterized it by inducing G1/S phase cell cycle arrest through treatment with Palbociclib and hydroxyurea. The effects of cell cycle dysregulation on tailbud development were also explored. Overexpression of CDKN1A/p21, a cyclin- dependent kinase inhibitor, was found to induce G1 cell cycle arrest in tailbud cells and disrupt development by forcing additional cells to invade into the notochord. Our sensor provides new evidence for the hypothesis that G1 cell cycle arrest induces invasive behavior and lays the foundation for understanding the relationship between cell cycle regulation and invasion, a concept that is critical to our understanding of cancer metastasis.

 

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ISEF 简介

英特尔国际科学与工程大奖赛，简称 "ISEF"，由美国 Society for Science and the Public（科学和公共服务协会）主办，英特尔公司冠名赞助，是全球规模最大、等级最高的中学生的科研科创赛事。ISEF 的学术活动学科包括了所有数学、自然科学、工程的全部领域和部分社会科学。ISEF 素有全球青少年科学学术活动的“世界杯”之美誉，旨在鼓励学生团队协作，开拓创新，长期专一深入地研究自己感兴趣的课题。

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英特尔 ISEF 学术活动详细介绍

英特尔 ISEF 全程指导方案

· 数学 · 物理 · 化学 · 生物 · 计算机 · 工程 ·

学科简介：细胞与分子生物学 CELLULAR AND MOLECULAR BIOLOGY

This is an interdisciplinary field that studies the structure, function, intracellular pathways, and formation of cells. Studies involve understanding life and cellular processes specifically at the molecular level.

Subcategories:

Cell Physiology (PHY): The study of the cell cycle, cell function, and interactions between cells or between cells and their environment. In general, projects could address physiology of membrane transport, neuron transmission, muscle contraction, the digestion of food, circulation of blood, contraction of muscles, or movement and production of nutrients in plant cells.In general, projects could address physiology of membrane transport, neuron transmission, muscle contraction, the digestion of food, circulation of blood, contraction of muscles, or movement and production of nutrients in plant cells.

Cellular Immunology (IMM): The study of the structure and function of the immune system at the cellular level. This includes investigations of innate and acquired (adaptive) immunity, the cellular communication pathways involved in immunity, cellular recognition and interactions between antigens and antibodies.

Genetics (GEN): The study of molecular genetics focusing on the structure and function of genes at a molecular level.genes, genetic variation, and heredity in living cells. These projects explore the consequences of genome variation on human cell biology, and thus gene function in health and disease.  Furthermore, projects may study the impact of naturally-occurring and engineered genome mutations in human iPS cells, their differentiated derivatives, and other cell types.

Molecular Biology (MOL): The study of biology at the molecular level. Chiefly concerns itself with understanding the interactions between the various systems of a cell, including the interrelationships of DNA, RNA and protein synthesis and learning how these interactions are regulated, such as during transcription and translation, the significance of introns and exons or coding issues.

Neurobiology (NEU): The study of the structure and function of the nervous system at the cellular or molecular level. This area focuses on the study of cells of the nervous system and the organization of these cells into functional circuits that process information and mediate behavior. Some neurobiology focuses on the molecular structures of the brain and nervous system. Larger complete systems, like the function and structure of the cerebral cortex, may be studied. Projects can look at biological factors that impact learning or mood, or how early genetic material develops into various areas of the brain.

OTH Other (OTH): Studies that cannot be assigned to one of the above subcategories. If the project involves multiple subcategories, the principal subcategory should be chosen instead of Other.